Micronuclei in lymphocytes of horses and pigs after in vitro irradiation

Abstract

Within a comprehensive study conducted on the Bosnian- Herzegovinia mountain horse breed, a research on the relative frequency of micronuclei (MN) after in vitro horse blood irradiation was carried out. Experiments on cytogenetic dosimetry were also conducted on pigs. The results of MN test were presented in terms of MN in binucleate (BN) cells. In control samples the percent of MN is very low in relation to irradiated samples where a higher number of MN cells was asserted, as well as a higher number of MN in BN cells. We could also observe that a number of BN cells with MN is proportionally increased with the radiation dosage. The MN test could provide additional data on the level of radiation damage (depletion) in the lymphocytes of the peripheral blood in domestic animals.