Abstract
Previous micromotor-based biosensing studies used to functionalize the surface of the micromotor with specific molecular probes for binding of target analyte, thus limiting the use of the micromotor for the specific target. In contrast, here, we introduce a novel approach of using a nonfunctionalized micromotor as a generic cargo carrier being able to perform label-free and dynamic loading, transport, and release of functionalized beads. Hence, such an approach enables one to use the same micromotor system for sensing of varying targets via different commercially available functionalized beads, demonstrating the use of micromotors as a practical and versatile means for biosensing. We have also introduced a simplified microfluidic design that can be used for immunosensing or DNA binding tests without necessity for complicated fluid handling (buffer exchange, washing, etc.) steps. We expect this approach to open up new realizations of simplified and generic biosensing platforms.
Highlights
Previous micromotor-based biosensing studies used to functionalize the surface of the micromotor with specific molecular probes for binding of target analyte, limiting the use of the micromotor for the specific target
A 10 μm-diameter Ni-Au-coated Janus particle was used as a micromotor within an indium tin oxide (ITO)-sandwiched microchamber wherein the bottom ITOcoated glass slide was covered with thin silicon dioxide layer to suppress adsorption of particles onto the substrate[27]
We have successfully demonstrated the ability to perform labelfree and dynamic manipulation and transport of biotin-coated beads used as cargo via a JP acting as an active carrier
Summary
Previous micromotor-based biosensing studies used to functionalize the surface of the micromotor with specific molecular probes for binding of target analyte, limiting the use of the micromotor for the specific target. We demonstrate a generic approach using a nonlabeled micromotor that can selectively load, transport, and release functionalized beads (i.e., treated as cargo) singularly controlled by an external alternating electric field.
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