Micromorphometric parameters of the thyroid gland and antioxidant status in rats with acute radiation damage against the background of the use of DAFS-25 and MONCLAVIT-1

Abstract

The search for formulations of radioprotective agents with different mechanisms of action is one of the promising areas in radiobiology. One of the components of the body's antioxidant system are glutathione peroxidases, selenium-dependent proteins capable of neutralizing reactive oxygen species and inhibiting lipid peroxidation. In addition, selenium is involved in the metabolism of thyroid hormones and the maintenance of cellular homeostasis of thyrocytes. The thyroid gland is involved in the regulation of metabolism, and also affects the functioning of many body systems. Consequently, the course and outcome of radiation pathology will depend on the functional state of the thyroid gland. Iodine is required for the synthesis of thyroid hormones. DAFS-25 is a feed additive for farm animals containing diacetophenonyl selenide (mass fraction of selenium is 25%). Monclavit-1 is a veterinary drug containing iodine in the form of a polymer complex. The combined use of selenium and iodine as a radioprotective agent is currently poorly understood. Thus, the purpose of this work was to evaluate changes in the concentration of malondialdehyde and the functional state of the thyroid gland in acute radiation injury against the background of the use of DAPS-25 and Monclavit-1.In order to conduct the experiment on the principle of pairs of analogues, 5 groups of animals were formed, 10 individuals each. The determination of the concentration of malondialdehyde was carried out by the colorimetric method. The material selected for histological examination was fixed in a formalin solution, then dehydrated, paraffinized, and stained with hematoxylin-eosin.The use of DAFS-25 and Monclavit-1 contributes to a decrease in the concentration of malondialdehyde in the blood of irradiated animals and a relative restoration of the histological structure of the thyroid gland on the 30th day of the experiment.