Abstract
The injection of holo-aequorin into embryos at the one-cell stage, along with the use of a simple photomultiplier tube or luminescence imaging system, allows transient localized elevations of free cytosolic Ca(2+) to be recorded and observed during the first 24 h of zebrafish development. The technique for loading dechorionated or intact one-cell stage zebrafish embryos with holo-aequorin is described here.
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