Abstract
We demonstrate a nonintrusive optical method for microfluidic detection and analysis based on evanescent wave sensing. The device consists of a planar thin-film waveguide integrated with a microfluidic chip for directed surface flow. Microliter droplets are electronically transported and positioned over the waveguide surface by thermocapillary actuation. The attenuated intensity of propagating modes is used to detect droplet location, to monitor dye concentration in aqueous solutions, and to measure reaction rates with increasing surface temperature for a chromogenic biochemical assay. This study illustrates a few of the capabilities possible by direct integration of optical sensing with surface-directed fluidic devices.
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