Abstract
Microfluidic analytical system was developed based on annular flow of phase separation multiphase flow with a ternary water-hydrophilic/hydrophobic organic solvent solution. The analytical system was combined with on-line luminol chemiluminescence detection for catechin analysis. The water (10 mM phosphate buffer, pH 7.3)-acetonitrile-ethyl acetate mixed solution (3:8:4, volume ratio) containing 60 μM luminol and 2 mM hydrogen peroxide as a carrier was fed into the capillary tube (open-tubular fused-silica, 75 μm inner diameter, 110 cm effective length) at a flow rate of 1.0 μL·min-1. The carrier solution showed stable chemiluminescence as a baseline on the flow chart. Eight catechins were detected as negative peaks for their antioxidant potential with different detection times. The system was applied to analyze the amounts of catechin in commercially available green tea beverages.
Highlights
Fluidic flow in a tube can be typically classified into one of two groups: homogeneous single-phase flows, which do not have a liquid-liquid interface, and immiscible multiphase flows, which have a liquid-liquid interface [1]
Microfluidic analytical system was developed based on annular flow of phase separation multiphase flow with a ternary water-hydrophilic/hydrophobic organic solvent solution
We have recently developed a method of generating a multiphase flow using two-phase separation mixed solvent solutions [10] [11] [12] [13]
Summary
Fluidic flow in a tube can be typically classified into one of two groups: homogeneous single-phase flows, which do not have a liquid-liquid interface, and immiscible multiphase flows, which have a liquid-liquid interface [1]. When mixed solvent solutions, such as a water-hydrophilic/hydrophobic organic solvent solution, are delivered into a microspace, such as a capillary tube and a microchannel on a microchip, phase transformation of the mixed solvent solution occurs in the microspace with changes in temperature and/or pressure, leading to a kinetic liquid-liquid interface. These new types of multiphase flows are called “phase separation multiphase flows”. The present fluidic analytical system was used to detect eight catechins with each detection times on the CL profile and further analyze the amounts of catechin in commercially available green tea beverages
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