Abstract
Glucose, glutamate and lactate oxidase as well as glutaminase were covalently immobilized on thin-film metal electrodes employing a polyimine gel obtained by polymerization of amines and aldehydes. The gel matrix having ion-exchange properties serves an an excellent suppression layer for electrochemically interfering compounds. A glutamine sensor was obtained using the glutamate sensor in combination with highly purified immobilized glutaminase from Escherichia coli, pig kidney and Bacillus subtilis. Sensor thus obtained showed rapid response, high stability, excellent suppression of interfering substances and short regeneration time. These sensors were designed for fermentation control in bioreactors using flow-injection-analysis device (FIA). The sensors were also applied for assaying in plasma and whole blood.
Sign-in/Register to access full text options 
Free) 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
