Abstract
Amperometric membrane glucose and lactate biosensors are investigated. 'Sandwich'-type membranes are used with an enzyme (glucose oxidase or lactate oxidase) immobilized between two membrane layers. A new type of catalytic electrode made from pyrolysed CoTMPP (cobalt-tetramethoxyphenylporphyrin) is used for detection of H 2O 2 generated by the enzymatic reaction. The glucose biosensor is used for the determination of glucose concentrations up to 2 mM/l in water solutions and in diluted blood serum samples. The biosensor can be used for more than 100 days. Concentrations of Li-lactate up to 1.5 mM/l in water solutions are determined with the lactate biosensor.
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