Abstract
Polyacrylamide gel electrophoresis is widely applied in modern biochemical and genetical studies for separating proteins and isozymes [ 1,2]. The techniques involve polymerization of the gel in tubes or in plates, both variants have their advantages. Tubes are suitable for assaying microamounts of protein, since the resolution can be increased by changing the tube diameter. However, difficulties arise when comparing the mobility of different samples due to differences arising in the polymerization of the gels at the start in different tubes. When plates are used the electrophorograms are easier to scan and the polymerization process in the zones of application of the samples is more standard, so that, in general, this variant of the technique is much more convenient for comparing the protein mobilities of different samples. However, since the electrophoresis takes place over a relatively large plate surface, microamounts become difficult to analyse. In order to combine the advantages of the two variants we propose the use of flat glass capillaries (1 x 2mm, 50 x 100~ and 25 x 50~). In the present study this modification was employ ed for determining the isozymes of single cells
Published Version
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