Abstract
An improved method of microdetermination of G6PD isoenzyme activity in human erythrocytes was developed by modification of previously reported procedures. The volume of blood samples was reduced from 2 ml to 20 μl. After hemolysis in 2% Triton X-100 and 0.1% β-mercaptoethanol, the samples were subjected to centrifugation and thin-layer isoelectric focusing in polyacrylamide gel (PAG-IEF). By comparison with the original method, excellent resolution was obtained by this more rapid and simple procedure.
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