Abstract

The calcium-sensitive, membrane-binding annexin, p68, has been crystallized from solutions of polyethylene glycol and ammonium sulfate. Our electron microscopy and X-ray diffraction data indicate that p68 crystals are tetragonal, in space group P4 1, and have unit cell dimensions of a = b = 68.4 A ̊ and c = 209.6 A ̊ . The mechanism of cystallization from polyethylene glycol involves a transition from a paracrystalline form to ordered crystals by lateral reordering of chains of molecules extended along the c axis. These chains are directional and might reflect a mechanism whereby the two different ends of (chains of) the p68 molecules interact with different membranes.

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