Abstract
ABSTRACTIn this study, we used 16S rRNA gene amplicons to describe the bacterial microbiota associated with oysters (Crassostrea virginica) and seawater collected from two sites in the Chesapeake Bay. The dominant bacterial groups included those belonging to the order Pelagibacteraceae, family Enterobacteriaceae, and genus Synechococcus. The microbiomes varied among oysters from the same site and between the two sites and months.
Highlights
In this study, we used 16S rRNA gene amplicons to describe the bacterial microbiota associated with oysters (Crassostrea virginica) and seawater collected from two sites in the Chesapeake Bay
Much work has effectively described the prevalence of human pathogens, such as Vibrio spp. [7,8,9,10], associated with oysters
Additional culture-independent profiling of bacterial species associated with oyster microbiomes will continue to improve our understanding of risk factors and mitigation strategies
Summary
We used 16S rRNA gene amplicons to describe the bacterial microbiota associated with oysters (Crassostrea virginica) and seawater collected from two sites in the Chesapeake Bay. Much work has effectively described the prevalence of human pathogens, such as Vibrio spp. Additional culture-independent profiling of bacterial species associated with oyster microbiomes will continue to improve our understanding of risk factors and mitigation strategies. We used a metagenomic approach (16S rRNA gene amplicons) to characterize the bacterial composition of C. virginica and water from the Chesapeake Bay. A total of 20 oysters and 12 water samples were collected between May and June 2016 from two sites (Manokin River and Chester River) in the Chesapeake Bay. At each collection event, five oysters and 1 liter of water were collected from each site.
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