Microbiological evaluation for antifungal activity of some metal oxides nanofillers incorporated into cold cured soft lining materials: clinical based study

Abstract

Objective: The aim of the current study was to evaluate the efficacy of addition of zirconium oxide (ZrO2), titanium oxide(TiO2), and silica oxide (SiO2) nanoparticles to cold-cured soft liner on adhesion of Candida albicans (CA). Material andMethods: Fifty-four patients had been selected and divided into three groups according to the modification of soft linerwith ZrO2, TiO2, and SiO2 nanoparticles (18 each of). Each patient received maxillary complete denture having threecavities, the cavities were lined using cold cured soft liner modified with different concentration (0%, 3%, and 7%) ofmetal oxide nanoparticles. On days 14 and 28, swaps were taken out from relining site and immediately cultured forfungal evaluation. The number of colonies were counted, data collected and explored for normality using Shapiro-Wilktest, logarithmic transformation of CA count was performed. Repeated and one-way ANOVA were used followed byTukey HSD. Independent-t test used to compare between CA counts at different periods. Results: The CA adhesionwas significantly decreased by the addition of ZrO2, TiO2 and SiO2 nanoparticles in comparison with control group, alsothe antifungal coverage increased with nanoparticles concentration increased (P<0.005). The highest CA count wasidentified in group SiO2 followed by ZrO2, while TiO2 showed the lowest CA count (P <0.001). Conclusion: Additionof different nanoparticles; ZrO2, TiO2 and SiO2 to cold-cured soft liner is an effective method for reducing CA adhesion.KEYWORDSAntifungal agent; Candida albicans; Denture liners; Nanoparticles.