Microbial transformation mechanisms of particulate organic carbon to mineral-associated organic carbon at the chemical molecular level: Highlighting the effects of ambient temperature and soil moisture

Abstract

Soil dissolved organic matter (DOM), an important component of active soil organic carbon (C), plays a crucial role in controlling C transformation. However, the role of DOM in mediating the conversion of particulate organic C (POC) into mineral-associated organic C (MAOC) at the molecular level remains unclear. In this study, the microbial conversion of POC to MAOC at the molecular level was investigated at different ambient temperatures and soil moisture levels. An increase in ambient temperature led to the conversion of POC to labile C, whereas MAOC increased with soil moisture. CHO, CHNO, and CHOS increased with soil moisture and temperature. The highest temperature (35 °C) promoted conversion of lignin/cram (carboxyl-rich alicyclic molecules)-like molecules into aromatic components with the highest intragroup transformation of DOM (74%). The highest soil moisture (60%) favoured the conversion of refractory substances into aliphatic/peptide and lipid components with the highest intergroup transformation (28%). Compared to fungi, bacteria were the main driving force for DOM transformation. Therefore, changes in ambient temperature and soil moisture control the microbial formation and transformation of different C fractions (i.e. POC and MAOC) via DOM differently at the molecular level.