Abstract
The hydrogen-based partial denitrification coupled with anammox (H2-PDA) biofilm system effectively achieves low-carbon and high-efficiency biological nitrogen removal. However, the effects and biological interaction mechanism of H2 flux with the H2-PDA system have not yet been understood. This study assessed the effects of H2 flux on interactions among anammox bacteria (AnAOB), denitrifying bacteria (DB), and sulfate-reducing bacteria (SRB) coexisting in a H2-PDA system. Results showed the simultaneous removal of 40mg/L ammonium nitrogen (NH4+-N) and 50mg/L nitrate nitrogen (NO3--N) in the H2-PDA system at a flux of 0.13-0.14 e- eq/(m2·d) without additional organic carbon. Candidatus_Brocadia was involved in H2 oxidation and was negatively associated with the heterotrophic Thauera genus (DB). The expression of nirS and dsrA was increased to 5.6×105 copies/gSS and 2.1×105 copies/gSS, respectively, with excessive H2 flux (0.17 e- eq/(m2·d). This study provides technical guidance for understanding and applying the H2-PDA technology for low-carbon wastewater treatment.
Published Version
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