Abstract
Chitin and protein-containing marine by-products (CPCMBPs), including crab shells, squid pens, and shrimp shells, were investigated as the sole carbon/nitrogen (C/N) source for prodigiosin (PG) production by Serratia marcescens TNU01 in a 250 mL Erlenmeyer flask and a 10 L bioreactor system. Among the used C/N source of CPCMBPs, squid pens powder (SPP) showed the most optimum PG productivity. Different ratios of chitin/protein combination were also used as the C/N sources for PG production. With a similar chitin/protein ratio (4/6) of squid pens, a significant PG productivity was achieved when the chitin/protein ratios were controlled in the range of 3/7–4/6. Maximum PG yield (3450 mg/L) by S. marcescens TNU01 was achieved in the bioreactor system containing 3 L medium of 1.75% SPP, 0.03% K2HPO4, and 0.05% MgSO4 at 25 °C for 12 h in dark. The results of in vitro bioassays reveal that the purified PG possesses acetylcholinesterase inhibitory activity and antioxidant as well as anticancer activities. This study suggests that squid pens may have the potential to be used for cost effective production of bioactive PG at a large-scale.
Highlights
Chitin and protein-containing marine by-products (CPCMBPs), such as squid pens, shrimp and crab shells, are obtained abundantly from the fishery processing industry [1,2,3,4,5,6]
CPCMBPs contain a high amount of protein and mineral salts, strong inorganic acid and alkali are used for demineralization and deproteinization of CPCMBPs to produce chitin, causing pollution due to acid or alkali liquid [4,7]
Squid pens powders were used for fermentation by different S. marcescens strains to compare their PG producing ability
Summary
Chitin and protein-containing marine by-products (CPCMBPs), such as squid pens, shrimp and crab shells, are obtained abundantly from the fishery processing industry [1,2,3,4,5,6]. CPCMBPs have high chitin content and were utilized earlier for the production of chitin. Bioprocessing, being environmentally-friendly, was established for conversion of CPCMBPs into chitin [8], and several other bioactive products were applied for nutritional, biotechnological, agricultural, biomedical, and pharmaceutical purposes [8,9,10,11,12,13,14,15]. CPCMBPs were used for the cost effective production of bioactive prodigiosin (PG) at a high level via microbial fermentation
Sign-in/Register to view highlights & summary Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
