Microbial Proteomics Approach for Sensitive Quantitative Predictions of MHC Binding Peptide from Taenia Ovis

Gomase Vs

doi:10.4172/2153-0602.1000121

Abstract

Taenia ovis is a tapeworm parasite with the adult stage of the parasite found in the intestines of dogs, while the intermediate or larval stage is found in the muscles of sheep, causes sheep measles. Peptide fragments of antigen protein can be used to select nonamers for use in rational vaccine design, and to increase the understanding of roles of the immune system in infectious diseases. Analysis shows MHC class II binding peptides of antigen protein from Taenia ovis are important determinants for protection of host from parasitic infection. In this assay, we used PSSM and SVM algorithms for antigen design and predicted the binding affinity of antigen protein having 254 amino acids, which shows 246 nonamers. Binding ability prediction of antigen peptides to Major Histocompatibility Complex (MHC) class I & II molecules is important in vaccine development against sheep measles.

Highlights

Taenia ovis are the smallest nematode parasite of sheep, are responsible for ovine cysticercosis (Sheep Measles), have an unusual life cycle, and are one of the most widespread and clinically important parasites in the world [1,2]
Taenia ovis antigen peptides are most suitable for subunit vaccine development, because with single epitope the immune response can be generated in a large population
Antigen protein from Taenia ovis is necessary for new paradigm of synthetic vaccine development and target validation [3,4,5]

Summary

Introduction

Taenia ovis are the smallest nematode parasite of sheep, are responsible for ovine cysticercosis (Sheep Measles), have an unusual life cycle, and are one of the most widespread and clinically important parasites in the world [1,2]. Taenia ovis antigen peptides are most suitable for subunit vaccine development, because with single epitope the immune response can be generated in a large population. This approach is based on the phenomenon of cross-protection, whereby infected with a mild strain and is protected against a more severe strain of the same. The phenotype of the resistant transgenic hosts includes fewer centers of initial infection, a delay in symptom development and low accumulation. Antigen protein from Taenia ovis is necessary for new paradigm of synthetic vaccine development and target validation [3,4,5]

Methods

Results

Conclusion