Microbial P limitation in tropical forest soils could be overestimated: Insight from a sorption experiment and a meta-analysis

Abstract

The prevailing paradigm for soil microbial activity in tropical forests is that microbial activity is limited by phosphorus (P) availability, and thus exogenous P addition stimulates organic matter decomposition. This idea has been testified by studies demonstrating that experimental P addition accelerates soil respiration. Contrary to this conventional view, we hypothesize that the increased rates of soil microbial respiration could be due to the release of organic material from the surface of soil minerals when P is added, because P competes with organic C for binding sites in soil particles. Here we performed a sorption experiment in a tropical evergreen forest in southern China, where P addition had previously been reported to stimulate soil respiration but suppressed leaf litter decomposition. P addition to soils significantly increased dissolved organic carbon (DOC) content, which was extracted immediately after P addition and under a cold temperature where microbial activity was suppressed. This result can explain why P addition stimulated soil respiration but not litter decomposition in our study site. Namely, P addition abiotically elevated microbially-available C through the release of organic matter from the soil mineral surface. We also conducted a meta-analysis using data obtained in forest ecosystems, demonstrating that previous studies have consistently reported that P addition led to higher response ratios of soil microbial respiration than litter decomposition. Our findings suggest that the prevailing paradigm (i.e., soil microbial activity in tropical forests is limited by P availability) might require re-evaluation.