Lack of P limitation on litter decomposition: a case study from long-term addition of phosphorus and nitrogen in three subtropical forests in southern China

Abstract

ABSTRACT Contrary to the widely accepted belief that elevated soil respiration after phosphorus (P) fertilization indicates P limitations for soil microorganisms in (sub)tropical forests, our previous studies have proposed that the elevated respiration results from the abiotically elevated availability of carbon (C), originating from competitive desorption from sorption sites on the surface of soil particles following P fertilization. In this study, we tested the impact of P fertilization (150 kg NaH2PO4-P ha−1 yr−1) on the decomposition of tea bags (Lipton Rooibos and Green teas) in three types of subtropical forests (primary, secondary, and planted forests) at the Dinghushan Biosphere Reserve, where previous findings reported increased soil respiration following P fertilization. If the traditional view holds true, early-stage litter decomposition should be stimulated by P fertilization, similar to the observed stimulation in soil respiration. Conversely, if this is not the case, it strongly reinforces our new hypothesis. Tea bags were employed to serve a twofold purpose: to assess the effects of P fertilization on litter decomposition within the soil, rather than on the soil surface, and to concentrate on the decomposition of early-stage litter. Our results demonstrated that P fertilization did not accelerate the decomposition of tea bags, indicating the exclusive enhancement of soil respiration without simultaneous stimulation of litter decomposition. This contradicts expectations if P were limiting microbial activity, reinforcing our hypothesis. In conclusion, our study reaffirmed the hypothesis that microbial activity in tropical forest soils is not primarily limited by P availability.