Abstract
Here a novel micro-tube bioreactor (MTB) was reported based on the high gas-permeability Teflon tube, realizing controllable and bubble-free oxygen supply with low shear stress during microbial cultivation. It was validated by cultivating six microbial strains in MTB and conventional shake flasks, where improved growth performance was achieved in MTB with regards to the incipient growth rate and terminal biomass accumulation. Typical fed-batch cultivation of Bacillus subtilis was conducted in MTB to enable high-density microbial cultivation, which has tripled in biomass accumulation from batch mode. Adaptive evolution against oxygen for Lactobacillus plantarum was performed through an 8-day cultivation process under escalating oxygen partial pressure from 5 % to 20 % in MTB, and remarkably improved growth performance was obtained in aerobic environment in comparison with the parent strain (with biomass accumulation increased by 130–150 %). MTB is demonstrated to be a promising platform for controllable bubble-free oxygen supply with automated and easy operation for microbial cultivation and adaptive evolution.
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