Abstract
Here a novel micro-tube bioreactor (MTB) was reported based on the high gas-permeability Teflon tube, realizing controllable and bubble-free oxygen supply with low shear stress during microbial cultivation. It was validated by cultivating six microbial strains in MTB and conventional shake flasks, where improved growth performance was achieved in MTB with regards to the incipient growth rate and terminal biomass accumulation. Typical fed-batch cultivation of Bacillus subtilis was conducted in MTB to enable high-density microbial cultivation, which has tripled in biomass accumulation from batch mode. Adaptive evolution against oxygen for Lactobacillus plantarum was performed through an 8-day cultivation process under escalating oxygen partial pressure from 5 % to 20 % in MTB, and remarkably improved growth performance was obtained in aerobic environment in comparison with the parent strain (with biomass accumulation increased by 130–150 %). MTB is demonstrated to be a promising platform for controllable bubble-free oxygen supply with automated and easy operation for microbial cultivation and adaptive evolution.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.