Abstract
Microbial hydroxylation of progesterone occurred in the biotransformation by Phomopsis sp.. The conversion products were purified by column chromatography with ether/EtOAc and characterized by spectroscopic methods including 1HNMR, 13C NMR, IR, UV and MS. Those conversion products were identified as 11α-hydroxyprogesterone (2), 11α,15β-dihydroxy progesterone (3),11α-dihydroxypreg-1,4-dien-3,20-dione (4), 6β,15βdihydroxypregesterone (5) and 7β,15βdihydroxypregesterone (6).
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