Abstract
Microbial hydroxylation of 2-dodecen-1-yl-succinic anhydride at non-activated carbon atoms has been described. A screening with fourteen fungi strains was carried out. Most of the microorganisms transformed the substrate into new products. Biotransformation conditions with Mortierella isabellina 212 were optimized. Optically active products of hydroxylation at C–10 and C–11 were isolated. Chemical structures of the unreacted optically active substrate and the products were determined by spectroscopic methods (1H NMR, 13C NMR, GCMS, IR). Non-enzymatic hydrolysis of the anhydride at different pH was studied and methods of detection of the anhydride and the forming acid were discussed.
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