Abstract
Azo dyes constitute the largest and most versatile class of synthetic dyes used in the textile, pharmaceutical, food and cosmetics industries and represent major components in wastewater from the industrial dying processes. Biological degradation of Amido Black (AB), an azo dye, by a lab isolate of Pseudomonas sp. was studied by varying the culture conditions. About 90% of AB (100ppm) degradation was observed within 45 h of incubation in nutrient broth medium at 28±2°C under static conditions. The effect of inoculum size, dye concentration and initial pH of the solution on AB degradation was analyzed. UV–Visible spectra and FTIR spectra of the dye samples before and after decolorization confirmed the ability of the Pseudomonas sp. to degrade AB. The kinetic parameters for biodegradation of AB were estimated using three kinetic models available in the literature. AB biodegradation was found to follow the pseudo-second order kinetic model and their correlation coefficients were found to be around 0.995–0.998. In addition, the phytotoxicity study revealed the degradation of AB into a non-toxic product by Pseudomonas sp.
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