Microbial characterization and degradation of linear alkylbenzene sulfonate in an anaerobic reactor treating wastewater containing soap powder

Abstract

The aim of this study was to evaluate the removal of linear alkylbenzene sulfonate (LAS) in an anaerobic fluidized bed reactor (AFBR) treating wastewater containing soap powder as LAS source. At Stage I, the AFBR was fed with a synthetic substrate containing yeast extract and ethanol as carbon sources, and without LAS; at Stage II, soap powder was added to this synthetic substrate obtaining an LAS concentration of 14±3mgL−1. The compounds of soap powder probably inhibited some groups of microorganisms, increasing the concentration of volatile fatty acids (VFA) from 91 to 143mgHAcL−1. Consequently, the LAS removal rate was 48±10% after the 156days of operation. By sequencing, 16S rRNA clones belonging to the phyla Proteobacteria and Synergistetes were identified in the samples taken at the end of the experiment, with a remarkable presence of Dechloromonas sp. and Geobacter sp.