Abstract
Combining a quantitative histochemistry method with the Firefly luciferase method we found that adenosine-triphosphate (ATP) could be accurately measured in 100 to 200 μg of aortic tissue. Trichloracetic acid solution was used for extraction of ATP and by so doing, weighing at the freezing point or neutralization by (potassium hydroxide) KOH in case of perchloric acid could be eliminated. Simultaneous assay of cyclic nucleotides was thus feasible. Contents of ATP and ADP in intima and media of aortic wall of rabbits, cows, and pigs and levels of these nucleotides in atherosclerotic lesions of aorta of rabbits fed a 1% cholesterol containig diet for 15 weeks were measured. Levels of adenine nucleotides in atherosclerotic lesions of rabbits were low compared to levels in intima of the aortic wall. Acquisition of these data is vital for monitoring changes in levels of adenine nucleotides in relation to pathological changes in tissues.
Published Version
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