Microarray profiling provides insights into mechanisms of mesenchymal stem cells-mediated immunomodulation of activated microglia

Abstract

Event Abstract Back to Event Microarray profiling provides insights into mechanisms of mesenchymal stem cells-mediated immunomodulation of activated microglia Shinsmon Jose1*, Soon C. Chan2, King-Hwa Ling3, Abhi Veerakumarasivam3 and Sharmili Vidyadaran1 1 Universiti Putra Malaysia, Neuroinflammation Group, Immunology Laboratory, Department of Pathology, Faculty of Medicine Health Sciences, Malaysia 2 Perdana University , Graduate School of Medicine (PUGSOM), Malaysia 3 Universiti Putra Malaysia, Medical Genetics Laboratory, Faculty of Medicine and Health Sciences, Malaysia Mesenchymal stem cell’s (MSC) potential to exert an immunomodulatory effect on activated microglia is known for over a decade. However, the mechanism of immunomodulation is poorly understood. Here, we employ a microarray [Agilent] to first define the genes expressed in microglia following an inflammatory stimuli [LPS] and secondly to elucidate the effects exerted by MSC on those genes. Transcriptome level responses to LPS and MSC were determined based on differentially expressed gene (DEG) profile of Refseq annotated genes compared to unstimulated microglia (p<0.05, FC>2). A total of 428 genes were differentially expressed in LPS-stimulated microglia compared to 449 in activated co-culture. Gene ontology of DEGs in these two groups using DAVID Bioinformatics online tool were similar. However, a close examination of DEGs revealed that co-culture with MSC downregulated the expression of inflammatory molecules such as lipocalin-2 (percentage reduction; 9), TNF (19), CXCL10 (44), CD40 (32) and several other pro-inflammatory molecules which were upregulated in LPS-stimulated microglia. In addition, co-culture with MSC induced the expression of anti-inflammatory genes such as TGM2 (fold increase; 3), TGFß (3), TIMP (18) and IL1ra (12) compared to LPS-stimulated microglia. By using this non-conventional approach of microarray data analysis, we have obtained a snapshot view of the genes expressed by microglia in response to an inflammatory stimulus that are modulated by MSC. Keywords: Gene Expression, Immunomodulation, Mesenchymal Stem Cells, Microglia, Microarray Conference: 14th Meeting of the Asian-Pacific Society for Neurochemistry, Kuala Lumpur, Malaysia, 27 Aug - 30 Aug, 2016. Presentation Type: YIC06: Young Investigator Colloquium 6 Topic: 14th Meeting of the Asian-Pacific Society for Neurochemistry Citation: Jose S, Chan SC, Ling K, Veerakumarasivam A and Vidyadaran S (2016). Microarray profiling provides insights into mechanisms of mesenchymal stem cells-mediated immunomodulation of activated microglia. Conference Abstract: 14th Meeting of the Asian-Pacific Society for Neurochemistry. doi: 10.3389/conf.fncel.2016.36.00076 Copyright: The abstracts in this collection have not been subject to any Frontiers peer review or checks, and are not endorsed by Frontiers. They are made available through the Frontiers publishing platform as a service to conference organizers and presenters. The copyright in the individual abstracts is owned by the author of each abstract or his/her employer unless otherwise stated. Each abstract, as well as the collection of abstracts, are published under a Creative Commons CC-BY 4.0 (attribution) licence (https://creativecommons.org/licenses/by/4.0/) and may thus be reproduced, translated, adapted and be the subject of derivative works provided the authors and Frontiers are attributed. For Frontiers’ terms and conditions please see https://www.frontiersin.org/legal/terms-and-conditions. Received: 04 Aug 2016; Published Online: 11 Aug 2016. * Correspondence: Dr. Shinsmon Jose, Universiti Putra Malaysia, Neuroinflammation Group, Immunology Laboratory, Department of Pathology, Faculty of Medicine Health Sciences, Serdang, Selangor, Malaysia, josesn@ucmail.uc.edu Login Required This action requires you to be registered with Frontiers and logged in. To register or login click here. Abstract Info Abstract The Authors in Frontiers Shinsmon Jose Soon C Chan King-Hwa Ling Abhi Veerakumarasivam Sharmili Vidyadaran Google Shinsmon Jose Soon C Chan King-Hwa Ling Abhi Veerakumarasivam Sharmili Vidyadaran Google Scholar Shinsmon Jose Soon C Chan King-Hwa Ling Abhi Veerakumarasivam Sharmili Vidyadaran PubMed Shinsmon Jose Soon C Chan King-Hwa Ling Abhi Veerakumarasivam Sharmili Vidyadaran Related Article in Frontiers Google Scholar PubMed Abstract Close Back to top Javascript is disabled. Please enable Javascript in your browser settings in order to see all the content on this page.