Abstract
Neutral urinary 17-ketosteroids are measured by a simpler colorimetric procedure covering 24 or more determinations in 4 to 5 hours' work. Small volumes of urine and inexpensive laboratory equipment are required. Urine specimens are hydrolyzed at 90 C using diluted formaldehyde to destroy the pigments. The advantage of uncolored extracts makes the preparation of urine blanks unnecessary. To shorten time the Zimmermann reaction was modified keeping the m-dinitrobenzene and potassium hydroxide at 37 C during 25 minutes. Recovery experiments varied between 91.4 and 101.4 per cent with an average of 96.1 per cent. An excellent reproducibility of results are obtained with duplicated samples of urine assayed. The 17-ketogenic steroids are made simultaneously by previous oxidation with sodium bismuthate in a water bath at 70 C during 10 minutes, thus enabling us to save a considerable amount of time.
Published Version
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