Abstract
A novel method that can measure the fluid temperature in microscopic scale by measuring the fluorescence polarization is described in this paper. The measurement technique is not influenced by the quenching effects which appears in conventional LIF methods and is believed to show a higher reliability in temperature measurements. Experiment was performed using a microchannel flow and fluorescent molecule probes, and the effects of the fluid temperature, fluid viscosity, measurement time, and pH of the solution on the measured fluorescence polarization degree are discussed to understand the basic characteristics of the present method. The results showed that fluorescence polarization is considerably less sensible to these quenching factors. A good correlation with the fluid temperature, on the other hand, was obtained and agreed well with the theoretical values confirming the feasibility of the method.
Highlights
Microfluidic device are considered to be powerful tools in the fields of chemistry, biology and medicine in terms of enhancing the accuracy, sensitivity, and throughput and reducing the cost and time of the diagnoses and analysis
A novel fluid temperature measurement method based on the fluorescence polarization measurement is proposed in this study
Fluorescent molecules are strongly pH dependent [9] and the effect of the fluid pH on the fluorescent intensity and polarization are evaluated in this study
Summary
Fabrication of Stainless Steel Mold Using Electrochemical Fabrication Method for Microfluidic.
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