Micro propagation of Adiantum Capillus plant through a culture of plant spores

Abstract

This experiment was conducted in Biotechnology research center BTRC for the purpose of studying the possibility of propagate Adiantum Capillus plant by using spores through plant tissues culture technology, which is one of the endangered plants in Libya. MS media was used in this study supplemented with some growth regulators (cytokines) of benzyl adenine BA and Kinten K at different concentrations (0.0, 0.5, 1.0, 2.0) mg / l. The results indicate that the growth of spores represented by the increase in the length and size of the vegetative masses in the MS nutritional medium equipped with BA growth regulator at a concentration of 2.0 mg / l is significantly higher than other treatments. The obtained plants were adapted by using growth medium of the Betmos and sand mixture in a ratio of 1: 2 in the growth room. The branches and roots formed when the plant moved to the greenhouse in the same medium of development