Abstract
A rapid and efficient method for the quantitative determination of sterols containing a Δ 22-unsaturation has been developed. The technique consists of a controlled periodate-permanganate oxidation of the C 27, C 28, or C 29 sterol, extraction of the acidic fragments, and analysis by gas-liquid chromatography of the C 5, C 6, or C 7 carboxylic acid cleavage product using a 10% diethylene glycol succinate + 2% phosphoric acid liquid phase. Oxidation of 22-dehydrocholesteryl acetate and 7,22-bisdehydrocholesteryl acetate produced isovaleric acid, and oxidation of ergosterol and stigmasterol gave α-methylisovaleric acid and α-ethylisovaleric acid, respectively. Except for the stigmasterol oxidation, the yields were high. As expected, no short-chain acidic products could be detected from cholesterol, 7-dehydrocholesterol, or lathosterol.
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