Abstract
Electrophoresis 2015, 36, 1172–1178. DOI: 10.1002/elps.201400569 Islets of Langerhans are responsible for maintaining glucose homeostasis through regulated secretion of hormones and other factors. It is hypothesized that amino acids secreted from islets play a critical role in cell functionality and viability. To characterize the potential roles that these small molecules may play in islet physiology, derivatization and MEKC separation conditions were optimized using D-Norvaline as an internal standard. The cover image shows an electropherogram corresponding to the separation of amino acids released from 25 islets incubated in 3 mM glucose. Quantification of 14 amino acids was performed with LODs ranging from 0.2–7 nM. The effects of glucose and 2,4-dinitrophenol on amino acid secretions from islets were tested and a suppressive effect of glucose on GABA release was observed, likely acting through ATP inactivation of glutamate decarboxylase.
Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
