Abstract
Sphingosine 1-phosphate (S1P) is an intercellular signaling molecule present in blood. Erythrocytes have a central role in maintaining the S1P concentration in the blood stream. We previously demonstrated that S1P is exported from erythrocytes by a glyburide-sensitive S1P transporter. However, the gene encoding the S1P transporter in erythrocytes is unknown. In this study, we found that the mouse erythroid cell line, MEDEP-E14, has S1P export activity and exhibits properties that are consistent with those of erythrocytes. Using microarray analysis of MEDEP-E14 cells and its parental cell line, E14TG2a, we identified several candidate genes for S1P export activity. Of those genes, only one gene, Mfsd2b, showed S1P transport activity. The properties of S1P release by MFSD2B were similar to those in erythrocytes. Moreover, knockout of MFSD2B in MEDEP-E14 cells decreased S1P export from the cells. These results strongly suggest that MFSD2B is a novel S1P transporter in erythroid cells.
Highlights
Sphingosine 1-phosphate (S1P) is a lipid mediator that is important for various cellular functions, such as in immunity[1,2], vascularization[3], and bone homeostasis[4]
We found that the mouse erythroid cell line MEDEP-E14 has S1P export activity that is similar to that in erythrocytes
S1P transport activity was detected in CHO cells that were overexpressing mouse sphingosine kinase 1 (CHO/ SPHK1 cells) when an S1P transporter gene was expressed in the cells[13]
Summary
Sphingosine 1-phosphate (S1P) is a lipid mediator that is important for various cellular functions, such as in immunity[1,2], vascularization[3], and bone homeostasis[4]. We found that the mouse erythroid cell line MEDEP-E14 has S1P export activity that is similar to that in erythrocytes. We examined whether S1P export from MEDEP-E14 cells shows the same characteristics of S1P transport activity observed in erythrocytes.
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