Abstract
Nephrotic syndrome is characterized by clinical manifestations, including proteinuria, hypoalbuminemia, hyperlipidemia, and edema. Hydrothorax is a rare but serious complication caused by enhanced fluid exudation due to hypoalbuminemia and changes in capillary permeability. In this study, the methylation of the SHOX-2, DAPK1, RAR-beta, and mir-37 genes was assessed in 35 patients with nephrotic syndrome and hydrothorax using DNA extracted from pleural fluid and urine. Methylation was determined using real-time PCR. The PCR results showed no methylation of SHOX-2, RAR-beta, DAPK1, or Mir-375. The amplification curves show an exponential increase in the signal and a stable plateau, indicating successful DNA amplification. The absence of methylation confirmed the high specificity of the method for detecting unmethylated sequences. These results highlight the need for further research to understand the epigenetic mechanisms of gene expression and to develop new therapeutic approaches aimed at modulating DNA methylation and restoring normal gene regulation.
Published Version
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