Methylation of adiponectin gene promoter in patients with type 2 diabetes mellitus and prediabetes

Abstract

Objective To investigate the levels of adiponectin (APN) and its gene promoter methylation in patients with type 2 diabetes mellitus and prediabetes. Methods Eighty patients diagnosed with type 2 diabetes mellitus between January 2015 and August 2016 (diabetes group) , and 50 patients examined with prediabetes (prediabetes group) and 50 healthy subjects (control group) in our hospital were included in this study. The carotid intima media thickness (CIMI) and serum APN were compared between the groups. The methylation rate of APN gene in each group was determined and statistically analyzed by methylation-specific polymerase chain reaction (MSP) . Results Compared with the control group, the FPG, TC, LDL-C and HOMA-IR were significantly higher in the diabetes group and prediabetes group, whereas the HDL-C was significantly lower, and there were statistically significant differences (P<0.05) . There were statistically significant differences in the above indicators between the diabetes group and prediabetes group (P<0.05) . Compared with the prediabetes group, the TG and CIMI were significantly higher in the diabetes group, whereas the FINS and HOMA-IR were significantly lower, and the differences were statistically significant (P<0.05) . The serum APN levels in the diabetes group, prediabetes group and the control group were (6.29±2.37) mg/L, (10.73±2.51) mg/L and (15.85±3.56) mg/L, respectively, and there was statistically significant differences between the three groups (P<0.05) . The methylation rates of APN gene promoter in the three groups were 77.50%, 60.00% and 34.00%, respectively. There was statistically significant difference between the groups (P<0.05) . In the diabetes group and prediabetes group, the serum APN in the patients with methylation of APN gene promoter was significantly lower and CIMI was higher than those in the patients without methylation of APN gene promoter (all P<0.05) . Conclusion Type 2 diabetes mellitus and prediabetes may reduce the serum APN level by methylation of APN gene promoter. Key words: Diabetes mellitus, type 2; Adiponectin; Methylation