Abstract
SUMMARYAmoebae of the Colonia isolate ofPhysarum polycephalumproduce plasmodia within individual amoebal clones.This paper reports the derivation from the Colonia strainC50 of a strainCL(Colonia Leicester) which produces plasmodia in clones with high efficiency and which completes the life cycle reliably and repeatedly in single clones. The derivation of a lineCLd (CLdelayed plasmodium formation) is described and, usingCLd, the construction of the isogenic heterothallic strainsmt1;CLandmt2;CL.The above strains provide a system for the isolation and genetic analysis of mutants ofP. polycephalumwithin a uniform genetic background, in particular mutants expressed in the plasmodium.Using this system two auxotrophic mutants have been isolated. Preliminary genetic analysis has shown that they are due to single gene mutations.
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