Methods for studying planar cell polarity

Abstract

Planar cell polarity (PCP) is the polarity of epithelial cells in the plane orthogonal to the apical–basal axis, and is controlled by a partially defined signaling system [54,76]. PCP related signaling also plays roles in cell migration, tissue re-organization and stem cell differentiation during embryonic development, and later, in regeneration and repair [29,58]. Aberrant signaling has been linked to a broad range of pathophysiologies including cancer, developmental defects, and neurological disorders [29,58,89]. The deepest mechanistic insights have come from studies of PCP in Drosophila[53,76]. In this chapter we review tools and methods to study PCP signaling in Drosophila epithelia, where it was found to involve asymmetric protein localization that is coordinated between adjacent cells. Such signaling has been most extensively studied in wing, eye, and abdomen, but also in other tissues such as leg and notum [1,45]. In the adult fly, PCP is manifested in the coordinated direction of hairs and bristles, as well as the organization of ommatidia in the eye. The polarity of these structures is preceded by asymmetric localization of PCP signaling proteins at the apical junctions of epithelial cells. Based on genetic and molecular criteria, the proteins that govern PCP can be divided into distinct modules, including the core module, the Fat/Dachsous/Four-jointed (Fat/Ds/Fj) module (often referred to as the ‘global’ module) as well as tissue specific effector modules [36,54,91]. Different tissues and tissue regions differ in their sensitivity to disturbances in the various modules of the PCP signaling system, leading to controversies about the interactions among the modules, and emphasizing the value of studying PCP in multiple contexts [45,62]. Here, we review methods including those generally applicable, as well as some that are selectively useful for analyses of PCP in eye (including eye discs), wing (including wing discs), pupal and adult abdomen, and the cuticle of larvae and embryos.