Abstract

AbstractThe effect of in vitro cold treatment (+6°C, 90 days) on selection of low‐temperature tolerant (LTT) mutants was investigated using X‐irradiated (15 Gy and 20 Gy) and non‐irradiated cell suspensions of Chrysanthemum morifolium cv. ‘Parliament’. Low temperature reduced significantly the number of regenerating calli, especially in the irradiated cultures. After the end of the cold stress 684, 197 and 54 plants were obtained from the 0 Gy, 15 Gy and 20 Gy treated cultures, respectively. Testing these plants in the greenhouse (+12 °C, winter low‐light) most of the early flowering variants were selected from the 15 Gy population.

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