Abstract
Methods and instruments to produce minute spots of ultraviolet light to realize selective irradiation of chromatin in living cells are discussed theoretically and checked experimentally. Two methods were developed: (1) lens method (focusing of ultraviolet light by means of quartz or fluorite lens), and (2) screen method (screening of ultraviolet light by means of a screen with minute opening). While the diffraction phenomena restrict the lower limit of the size of the irradiated spot, yet a spot as minute as 0.2μ in diameter with the lens method, and 1μ in diameter with the screen method, is obtainable. Biological material suitable for experimentation (certain protozoa, spermatozoa) is discussed briefly.
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