Abstract
BackgroundSpecimen preparation for in vitro study suffers a general lack of methodological homogeneity, as well as a lack of uniformity in the materials selected for fabricating them. This situation prevents comparisons between studies. When a specimen is not of dental origin it is not possible to study adhesion or bonding techniques realistically. The objective is to design and implement a method of specimen preparation that permits universal standardization for use in in vitro studies.Material and MethodsA metal stump of specified dimensions was designed and fabricated by hand. It was scanned, the data digitalized, perfecting and standardizing the dimensions. Ten human molars were adapted to the size and shape of a standard milling block. A Cerec 3D inLab Cerec milling unit was used to prepare the molars to match the digitalized model.ResultsTen specimens with identical dimensions were obtained.ConclusionsCAD-CAM technology allows the preparation of natural extracted teeth to be standardized and could be used to establish a reproducible method that would facilitate comparison between different in vitro studies, and reduce bias arising from variations in sample fabrication. Key words:Test ceramic, dentin analog, dentin model, dentin samples, methodology.
Highlights
One of the main problems facing in vitro research in the field of dentistry is the lack of homogeneity in methodology [1], a situation that limits the application of the concepts of evidence based dentistry
In addition to the standardization of samples, an attractive feature of these technologies is that they may be applied to specimens of dental origin
Using dental specimens that have been digitally standardized would greatly facilitate comparison of the data obtained between studies and the extrapolation of the results to other research situations
Summary
One of the main problems facing in vitro research in the field of dentistry is the lack of homogeneity in methodology [1], a situation that limits the application of the concepts of evidence based dentistry It prevents exact comparisons between different works of research, even when the same materials and techniques are used, and impedes extrapolation of the results obtained [2]. There are various methods for fabricating specimens by hand; all aim to be comparable within the study underway but the lack of standardization prevents extrapolation of the results to other centers or research teams who may wish to reproduce or develop the research [3] This does not favor the repetition of a study by another team that wishes to verify and contrast the data obtained, enlarge the sample size, or vary the bond materials used. Conclusions: CAD-CAM technology allows the preparation of natural extracted teeth to be standardized and could be used to establish a reproducible method that would facilitate comparison between different in vitro studies, and reduce bias arising from variations in sample fabrication
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