Methodology for Phylogenetic Tree Construction

Kudipudi Srinivas

doi:10.4172/jpb.s1000002

Abstract

It has been our endower to present and explore different steps involved in the construction of a phylogenetic tree using online services. The application of different bioinformatic tools is necessary for the realization of the desirable results. A stepwise construction strategy using on line ClustalW is proposed with suitable application. The method can hope fully will aid the reader to construct the phylogenetic tree. Construction of phylogenetic tree is useful for comparison of homology of functional proteins in one species and between species.

Highlights

Retrieving DNA sequence using major Nucleotide Sequence databases95th ISCA –Bioinformatics Section ISSN:0974-276X Volume S1: S005-S011(2008) - S005. GenBank (National Center for Biotechnology Information, NCBI) http:// www.ncbi.nlm.nih.gov/
Tree representation of the family history of set of sequences that share a common ancestor is called a Phylogenetic Tree
It has been our endower to present and explore different steps involved in the construction of a phylogenetic tree using online services

Summary

Retrieving DNA sequence using major Nucleotide Sequence databases

95th ISCA –Bioinformatics Section ISSN:0974-276X Volume S1: S005-S011(2008) - S005. GenBank (National Center for Biotechnology Information, NCBI) http:// www.ncbi.nlm.nih.gov/. Open the link at the EBI ClustalW server at www.ebi.ac.uk/clustalW/Index.html

Use Output format pull-down menu to set the selection of choice

Copy and Paste into the BLAST Searching window

Click the Format button

Choose NJ from Phylogenetic Tree

Results are saved

Conclusion