Methodical aspects of various biomorphs ornamental plants regeneration study

Abstract

The paper raises the problem of conservation, maintenance, rejuvenation and accelerated reproduction of plant objects in bioresource collections using green cutting propagation. The authors describe experimental methods of herbaceous and woody plants regeneration study in agricultural, physiological-biochemical and morphological directions. In agricultural direction the main factors are the timing and methods of green cutting, as well as the microecological conditions in which regeneration takes place (humidity, temperature of the air and substrate). The physiological and biochemical aspect concerns the use of various growth stimulants, including concentrations and timing for the treatment of unruly cuttings. The reliable positive effect of the growth stimulants in comparison with control natural regeneration can be proved by the t-criteria. The morphological aspect shows features of callusogenesis and rhizogenesis. The most profound analysis is possible when representatives of generic complexes, species and varieties of various ecological, geographical and genetic origin are involved in the experiment. A highly informative classification of callusogenesis and rhizogenesis proposed by N.F. Dovbysh, N.A. Oleinik and G.A. Kudina (1993) is described in detail; in this classification three types of callus implantation are described: circular, focal and continuous, as well as five types of localization and development of roots. Root formation can occur 1) in the zone of the node, 2) from the callus tissue, 3) from the side of the bud and leaf trail, 4) over the entire surface of the internode, as well as 5) mixed (from the tissues in the node zone and from callus tissue). Based on the long-term study of the regeneration of shrub and herbaceous ornamental plants, the authors of the paper propose one more type - in the entire surface of the lower cut.