Abstract
The paper raises the problem of conservation, maintenance, rejuvenation and accelerated reproduction of plant objects in bioresource collections using green cutting propagation. The authors describe experimental methods of herbaceous and woody plants regeneration study in agricultural, physiological-biochemical and morphological directions. In agricultural direction the main factors are the timing and methods of green cutting, as well as the microecological conditions in which regeneration takes place (humidity, temperature of the air and substrate). The physiological and biochemical aspect concerns the use of various growth stimulants, including concentrations and timing for the treatment of unruly cuttings. The reliable positive effect of the growth stimulants in comparison with control natural regeneration can be proved by the t-criteria. The morphological aspect shows features of callusogenesis and rhizogenesis. The most profound analysis is possible when representatives of generic complexes, species and varieties of various ecological, geographical and genetic origin are involved in the experiment. A highly informative classification of callusogenesis and rhizogenesis proposed by N.F. Dovbysh, N.A. Oleinik and G.A. Kudina (1993) is described in detail; in this classification three types of callus implantation are described: circular, focal and continuous, as well as five types of localization and development of roots. Root formation can occur 1) in the zone of the node, 2) from the callus tissue, 3) from the side of the bud and leaf trail, 4) over the entire surface of the internode, as well as 5) mixed (from the tissues in the node zone and from callus tissue). Based on the long-term study of the regeneration of shrub and herbaceous ornamental plants, the authors of the paper propose one more type in the entire surface of the lower cut.
Highlights
The paper raises the problem of conservation
biochemical aspect concerns the use of various growth stimulants
The reliable positive effect of the growth stimulants in comparison with control natural regeneration can be proved by the t-criteria
Summary
Агротехнический аспект изучения особенностей регенерации растений при зеленом черенковании подразумевает обработку данных методом многофакторного дисперсионного анализа, где в качестве факторов выступают сроки и способы черенкования, реже – микроэкологические условия проведения экспериментов. Поэтому при получении материала для черенкования из открытого грунта в период активной вегетации растений подбор оптимальных сроков черенкования различных объектов в конкретных географических пунктах весьма актуален. Как показали наши многолетние исследования, объектами которых являлись садовые розы и флоксы метельчатые, сорта-повторности должны или принадлежать к одной садовой группе, классу, разделу (например, розы группы флорибунда), или к одной группе по срокам цветения – последнее обусловливает приблизительно равную степень развития побегов, используемых для черенкования. В опытах по подбору оптимальных сроков (фактор А) и способов черенкования (фактор В) сортов флокса метельчатого в качестве повторностей использовались сорта среднего срока цветения. Таблица 1 – Укоренение сортов флокса метельчатого в условиях искусственного тумана
Sign-in/Register to view highlights & summary Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
