Abstract
Summary A method is described for measuring the nitrogenase activity of free-living N2-fixing bacteria. The amount of enzyme activity was determined by means of the acetylene-reduction assay using serum flasks with agar cultures of Azotobacter chroococcum . The influence was tested of the inoculum size, the substrate concentration (acetylene), and the size of the agar surface on the acetylene production. The nitrogenase activity was related to the protein content of the bacteria.
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