Abstract
Immunoaffinity purification coupled with high performance liquid chromatography with photodiode array detection was used to quantify microcystins in fish samples. The immunoaffinity purification method consisted of heat denaturation, pronase digestion, and Sep‐Pak C18 extraction together with subsequent purification using an immunoaffinity column. The immunoaffinity purification allowed precise analysis of microcystins‐RR, ‐YR, and ‐LR in fish samples by eliminating co‐extracted substances. An internal standard of [D‐Asp3]microcystin‐LR was used to improve precision and for the recovery corrections. The calibration curves for all 3 microcystins showed linear relationship at concentrations from 40 to 200 ng/g.
Published Version
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