Abstract
Background: Metoprolol has been widely used in the treatment of hypertension. The usage of drugs for cardiovascular diseases is highly required safety and effectiveness for patients. Therefore, a convenient method for the determination of metoprolol in plasma is necessary. Objectives: (1) To develope an HPLC method for quantification of metoprolol in human plasma and (2) To validate of the method. Materials and methods: human plasma. Results: Chromatographic conditions include: Eclipse XDB-C18 column, fluorescence detector, mobile phase: acetonitril : NaH2PO4 buffer (25 mmol/L, pH 3.0) with a solvent gradient program. The analytical method met current FDA guidelines for bioanalytical method validation. Conclusions: The method can be applied to determine metoprolol in biological fluids for pharmacokinetic and biocompatibility study. Key words: Metoprolol, plasma, HPLC
Published Version
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