Abstract
Methionine-enkephalin (MENK) is not an effective stimulus for inducing the superoxide (O-2) generation of human neutrophils, but it enhanced the O-2 generation stimulated by the chemotactic peptide N-formyl-methionyl-leucyl-phenylalanine (fMLP) or human recombinant interferon gamma (hrIFNγ) when the cells had been preincubated with MENK for 30 min at 37°C. The priming effect of MENK was not observed with stimulus such as lipopolysaccharide (LPS) or phorbol-12-myristate-13-acetate (PMA). The enhancing effect of MENK was abrogated if cells were treated with protein kinase C (PKC) inhibitor H7 (1-(5-isoquinolinylsulfonyl)-2-methylpiperazine) before fMLP or IFNγ. This finding indicates that MENK is a potent modulator of human neutrophils and can contribute to inflammatory process.
Published Version
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