Methanol extract of Origanum vulgare ameliorates autoimmune diabetes in mice

Abstract

Event Abstract Back to Event Methanol extract of Origanum vulgare ameliorates autoimmune diabetes in mice Ivana Stojanovic1*, Milica Vujicic1, Ivana Nikolic1, Vassiliki Kontogianni2, Pantelis Charisiadis2, Andreas Tzakos2 and Stanislava Stosic-Grujicic1 1 Institute for Biological Research "Sinisa Stankovic", University of Belgrade, Immunology, Serbia 2 University of Ioannina, Chemistry, Greece Type 1 diabetes (T1D), an autoimmune inflammatory disorder, develops as a consequence of pancreatic beta cell destruction mediated by various pro-inflammatory mediators. Since current T1D therapy mainly involves insulin replacement, constant efforts are being directed toward establishing novel therapeutic approaches. Plants or plant extracts are inexhaustible source of medicinal compounds. In this study we have used methanol extract of Origanum vulgare (oregano - OE) that has already shown immunomodulatory and cytoprotective properties in vitro. OE was prepared from oregano leaves by sequential extraction of four solvents of gradually increasing polarity (hexane, ethyl acetate, dichloromethane and methanol). To evaluate the effect of OE on diabetes development, the extract was administered intraperitoneally to C57BL/6 mice that were subjected to T1D induction by multiple low doses of steptozotocin (MLDS). Results indicate that 10-day OE treatment significantly reduced the incidence and the level of hyperglycemia in MLDS-treated mice and preserved physiological insulin concentration (measured by ELISA). Flow cytometric analysis revealed a reduction in CD4+ cell number within spleen and pancreatic lymph nodes of OE-treated mice. This was accompanied by a significant inhibition of ex vivo Th17-related IL-17 secretion, while proto-typical Th1 and Th2 cytokines (IFN-gamma and IL-4, respectively) remained unchanged. Further, the presence of FoxP3+ T regulatory cells in lymphoid tissues of OE-treated mice was similar to diabetic mice as well as the percentage of pro-inflammatory F4/80+CD40+ M1 and anti-inflammatory F4/80+CD206+ M2 macrophages. In conclusion, our results indicate that OE protected mice from diabetes development through direct blockade of IL-17-mediated lymphocyte response. Acknowledgements Project ON173013 Keywords: type 1 diabetes, Oregano, Cytokines, T regulatory cells, Macrophages Conference: 15th International Congress of Immunology (ICI), Milan, Italy, 22 Aug - 27 Aug, 2013. Presentation Type: Abstract Topic: Immune-mediated disease pathogenesis Citation: Stojanovic I, Vujicic M, Nikolic I, Kontogianni V, Charisiadis P, Tzakos A and Stosic-Grujicic S (2013). Methanol extract of Origanum vulgare ameliorates autoimmune diabetes in mice. Front. Immunol. Conference Abstract: 15th International Congress of Immunology (ICI). doi: 10.3389/conf.fimmu.2013.02.00382 Copyright: The abstracts in this collection have not been subject to any Frontiers peer review or checks, and are not endorsed by Frontiers. They are made available through the Frontiers publishing platform as a service to conference organizers and presenters. The copyright in the individual abstracts is owned by the author of each abstract or his/her employer unless otherwise stated. Each abstract, as well as the collection of abstracts, are published under a Creative Commons CC-BY 4.0 (attribution) licence (https://creativecommons.org/licenses/by/4.0/) and may thus be reproduced, translated, adapted and be the subject of derivative works provided the authors and Frontiers are attributed. For Frontiers’ terms and conditions please see https://www.frontiersin.org/legal/terms-and-conditions. Received: 18 Mar 2013; Published Online: 22 Aug 2013. * Correspondence: Dr. Ivana Stojanovic, Institute for Biological Research "Sinisa Stankovic", University of Belgrade, Immunology, Belgrade, 11108, Serbia, ivana@ibiss.bg.ac.rs Login Required This action requires you to be registered with Frontiers and logged in. To register or login click here. Abstract Info Abstract The Authors in Frontiers Ivana Stojanovic Milica Vujicic Ivana Nikolic Vassiliki Kontogianni Pantelis Charisiadis Andreas Tzakos Stanislava Stosic-Grujicic Google Ivana Stojanovic Milica Vujicic Ivana Nikolic Vassiliki Kontogianni Pantelis Charisiadis Andreas Tzakos Stanislava Stosic-Grujicic Google Scholar Ivana Stojanovic Milica Vujicic Ivana Nikolic Vassiliki Kontogianni Pantelis Charisiadis Andreas Tzakos Stanislava Stosic-Grujicic PubMed Ivana Stojanovic Milica Vujicic Ivana Nikolic Vassiliki Kontogianni Pantelis Charisiadis Andreas Tzakos Stanislava Stosic-Grujicic Related Article in Frontiers Google Scholar PubMed Abstract Close Back to top Javascript is disabled. Please enable Javascript in your browser settings in order to see all the content on this page.