Co-ligation of TLR3 and Dectin-1 agonists potentiates Th1 polarization capability on monocyte derived Langerhans cells in vitro

Abstract

Event Abstract Back to Event Co-ligation of TLR3 and Dectin-1 agonists potentiates Th1 polarization capability on monocyte derived Langerhans cells in vitro Ivan Rajkovic1*, Ana Dragicevic2, Sasa Vasilijic1, 2, Dragana Vucevic1, 2, Biljana Bozic3, Dusan Mihajlovic1, Milan Markovic1 and Miodrag Colic1 1 Medical Faculty of the Military Medical Academy, University of Defense, Serbia 2 Institute for Medical Research, Military Medical Academy, Serbia 3 Faculty of Biology, University of Belgrade, Institute for Physiology and Biochemistry, Serbia Langerhans cells represent the first dendritic cell subtype that encounters foreign pathogens due to its localization in skin and stratified mucosa. Their potential to initiate T-cell responses has recently been challenged. Since viral and fungal infections are among the most common in these tissues, we have studied effects of Toll-like receptor 3 (TLR3) and Dectin-1 agonists, polyinosinic:polycytidylic acid (poly I:C) and curdlan, respectively, on maturation of their in vitro counterparts, monocyte derived Langerhans cells (MoLCs). Immature (im)MoLCs were generated in the presence of GM-CSF, IL-4 and TGF-β1 and then treated with polyI:C, curdlan or their combination for 2 days. Both single or combined treatments elicited phenotypic maturation of imMoLCs with increased allostimulatory capacity. Poly I:C-treated MoLCs up-regulated both the production of IFN-γ and IL-17 by alloreactive CD4+ T cells while curdlan-treated MoLCs stimulated only the production of IFN-γ, compared to imMoLCs. Poly I:C+curdlan-treated MoLCs produced higher concentrations of IL-12 and IL-10 and stimulated more strongly the production of IFN-γ by alloreactive CD4+ T cells then curdlan-treated MoLCs. At the same time, poly I:C+curdlan-treated MoLCs produced more IL-23 but less IL-27 and were weaker stimulators of IL-17 by alloreactive CD4+ T cells then poly I:C-treated cells. Poly I:C+curdlan-treated MoLCs showed higher expression of CD40 and CCR7 then both poly I:C- and curdlan-treated MoLCs. Ligation of both TLR3 and Dectin-1 agonists on imMoLCs upregulated their Th1polarization capability compared to single agonists. This finding may suggest that MoLCs could be useful for immunotherapy. Acknowledgements This study was supported by the project of Ministry of Education, Science and Technological Development of the Republic of Serbia (ON175102). Keywords: Monocyte-derived Langerhans cells, Toll-Like Receptors, Dectin-1 receptors, T-helper cell polarization, Immunotherapy Conference: 15th International Congress of Immunology (ICI), Milan, Italy, 22 Aug - 27 Aug, 2013. Presentation Type: Abstract Topic: Innate immunity Citation: Rajkovic I, Dragicevic A, Vasilijic S, Vucevic D, Bozic B, Mihajlovic D, Markovic M and Colic M (2013). Co-ligation of TLR3 and Dectin-1 agonists potentiates Th1 polarization capability on monocyte derived Langerhans cells in vitro. Front. Immunol. Conference Abstract: 15th International Congress of Immunology (ICI). doi: 10.3389/conf.fimmu.2013.02.00124 Copyright: The abstracts in this collection have not been subject to any Frontiers peer review or checks, and are not endorsed by Frontiers. They are made available through the Frontiers publishing platform as a service to conference organizers and presenters. The copyright in the individual abstracts is owned by the author of each abstract or his/her employer unless otherwise stated. Each abstract, as well as the collection of abstracts, are published under a Creative Commons CC-BY 4.0 (attribution) licence (https://creativecommons.org/licenses/by/4.0/) and may thus be reproduced, translated, adapted and be the subject of derivative works provided the authors and Frontiers are attributed. For Frontiers’ terms and conditions please see https://www.frontiersin.org/legal/terms-and-conditions. Received: 11 Mar 2013; Published Online: 22 Aug 2013. * Correspondence: Dr. Ivan Rajkovic, Medical Faculty of the Military Medical Academy, University of Defense, Belgrade, 11000, Serbia, ivanrajkovic75@gmail.com Login Required This action requires you to be registered with Frontiers and logged in. To register or login click here. Abstract Info Abstract The Authors in Frontiers Ivan Rajkovic Ana Dragicevic Sasa Vasilijic Dragana Vucevic Biljana Bozic Dusan Mihajlovic Milan Markovic Miodrag Colic Google Ivan Rajkovic Ana Dragicevic Sasa Vasilijic Dragana Vucevic Biljana Bozic Dusan Mihajlovic Milan Markovic Miodrag Colic Google Scholar Ivan Rajkovic Ana Dragicevic Sasa Vasilijic Dragana Vucevic Biljana Bozic Dusan Mihajlovic Milan Markovic Miodrag Colic PubMed Ivan Rajkovic Ana Dragicevic Sasa Vasilijic Dragana Vucevic Biljana Bozic Dusan Mihajlovic Milan Markovic Miodrag Colic Related Article in Frontiers Google Scholar PubMed Abstract Close Back to top Javascript is disabled. Please enable Javascript in your browser settings in order to see all the content on this page.