Metallothionein expression on oysters ( Crassostrea cuculata and Crassostrea glomerata) from the southern coastal region of East Java.

Asus Maizar Suryanto Hertika,Defri Yona,Renanda Baghaz Dzulhamdhani Surya Putra,Erlinda Indrayani,Kusriani Kusriani

doi:10.12688/f1000research.17381.2

Asus Maizar Suryanto Hertika, Defri Yona + Show 3 more

Open Access

https://doi.org/10.12688/f1000research.17381.2

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Journal: F1000Research	Publication Date: Apr 30, 2020
Citations: 1	License type: CC BY 4.0

Affiliation: University of Brawijaya

Abstract

Background: This study aimed to analyse levels of heavy metals (Pb, Hg and Cd) in the aquatic body, gills and stomach of the oysters Crassostrea cuculata and Crassostrea glomerata, the metallothionein (MT) level in the gills and stomach of both oysters, and relationships between heavy metals level (Pb, Hg and Cd) in the gills and stomach to MT level in both species of oysters. Methods: The research method utilized was a descriptive method. The oyster samples were taken from three stations: Sendang Biru, Popoh and Prigi beaches. MT values were assessed using confocal laser scanning microscopy. The heavy metal levels were assessed using atomic absorption spectrophotometry method. Results: Both oyster heavy metal content obtained in the southern coastal waters exceeded the safe limit set by the State Minister of Environment No. 51 of 2004. In general, the expression of MT was found to be higher in stomach tissue compared to gill tissue. Conclusions: The levels of the heavy metals Pb, Hg, and has a strong relationship with MT levels in the gills and stomach in both types of oysters.

Highlights

This study aimed to analyse levels of heavy metals (Pb, Hg and Cd) in the aquatic body, gills and stomach of the oysters Crassostrea cuculata and Crassostrea glomerata, the metallothionein (MT) level in the gills and stomach of both oysters, and relationships between heavy metals level (Pb, Hg and Cd) in the gills and stomach to MT level in both species of oysters
This study aims to analyze heavy metal level (Pb, Hg, and Cd) in the tissues of oysters (Crassostrea cuculata and Crassostrea glomerata) and the coastal waters of the South coast of East Java to determine their relationship to MT (MT) expression
We observed physical and chemical water quality parameters that support the life of Crassostrea cuculata and Crassostrea glomerata, namely temperature, acidity, dissolved oxygen (DO) and salinity (Figure 1)

Summary

Introduction

This study aimed to analyse levels of heavy metals (Pb, Hg and Cd) in the aquatic body, gills and stomach of the oysters Crassostrea cuculata and Crassostrea glomerata, the metallothionein (MT) level in the gills and stomach of both oysters, and relationships between heavy metals level (Pb, Hg and Cd) in the gills and stomach to MT level in both species of oysters. The oyster samples were taken from three stations: Sendang Biru, Popoh and Prigi beaches. MT values were assessed using confocal laser scanning microscopy. The heavy metal levels were assessed using atomic absorption spectrophotometry method. Results: Both oyster heavy metal content obtained in the southern coastal waters exceeded the safe limit set by the State Minister of Environment No 51 of 2004. The expression of MT was found to be higher in stomach tissue compared to gill tissue. Conclusions: The levels of the heavy metals Pb, Hg, and has a strong relationship with MT levels in the gills and stomach in both types of oysters

Objectives

Methods

Results