Metallic Nanoparticle-Decorated Polydopamine Thin Films and Their Cell Proliferation Characteristics

Ferhunde Aysin,Mehmet Yilmaz,Asli Yilmaz

doi:10.3390/coatings10090802

Abstract

Plasmonic metal nanoparticle (NP)-decorated thin films of biobased and biocompatible polymers provide significant opportunities in various biomedical applications. Inspired from the adhesive proteins of the marine mussels, polydopamine (PDA) serves as a versatile, biocompatible, and simple thin-film material and enhances cell growth and proliferation. Herein, we report the fabrication of the gold NPs (AuNPs) or silver NPs (AgNPs)-deposited thin films of PDA and their employment in cell growth and proliferation. PDA thin film with its numerous functional groups enabled well-controlled adsorption of NPs. The number density of NPs was manipulated simply by tuning the deposition time. Cell viability test for human lung cancer (A549) and human colon cancer (CaCO2) cell lines indicated that a thin layer of PDA film remarkably enhanced the cell growth and proliferation. The lower number density of NPs for the 24 h of the culture time resulted in a higher proliferation rate. However, the increase in both the number density of NPs and culture time led to a decrease in cell growth.

Highlights

The adhesive proteins of marine mussels facilitate the attachment of these organisms to the rocks and ships in seawater [1]
We detected that the layer remarkably enhanced cell growth and proliferation
The NP decorated films indicated that cell growth and proliferation is highly dependent on both the type and the number density of NPs

Summary

Introduction

The adhesive proteins of marine mussels facilitate the attachment of these organisms to the rocks and ships in seawater [1]. Based on the previous studies, coating of the surfaces and nanoparticles with PDA helps to improve their biocompatibility and bioactivity, which will enhance their use in biological and biomedical applications [14,15,16] Plasmonic metallic nanostructures such as gold nanoparticles (AuNPs) and silver nanoparticles (AgNPs) having unique properties due to their interaction with electromagnetic radiation provide various opportunities in biological and medical applications [17,18,19]. The increase in both the number density of NPs and culture time led to a decrease in cell growth In light of these findings, we strongly believe that the proposed system can be used in diverse biological and medical applications

Synthesis of AgNPs and AuNPs

Characterization of NPs and Thin Films

Cell Culture and Cell Adhesion to Thin Films

UV-Vis absorption spectra andrepresentative representative images of gold

Biocompatibility Testing of Thin Films

Conclusions